Genotyping Methods and Protocols

Synopsis

This volume explores a broad range of different genotyping techniques. Genotyping: Methods and Protocols consists of chapters that cover numerous topics such as: an overview of multiplexed microsatellite analysis; High Resolution Melt analysis and TaqMan-based assays; in situ analysis of variants in single RNA molecules; the MassARRAY system and Molecular Inversion Probes; Pulsed Field Gel Electrophoresis, Paralogue Ratio Test, and Multiplex Ligation-Dependent Probe Amplification; long-range PCR combined with PacBio sequencing; Targeted Locus Amplification; Multilocus Sequence Typing and rapid SNP detection with pyrosequencing; and genotyping-by-sequencing for plant analysis. Finally, the volume concludes with a summary of pertinent points to describe genetic variation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Thorough and practical, Genotyping: Methods and Protocols is a valuable resource for anyone interested in learning more about the diverse field of genotyping.

Book details

Edition:

1st ed. 2017

Series:

Methods in Molecular Biology (Book 1492)

Author:

Stefan J. White and Stuart Cantsilieris

ISBN:

9781493964420

Related ISBNs:

9781493964406

Publisher:

Springer New York

Pages:

N/A

Reading age:

Not specified

Includes images:

Yes

Date of addition:

2021-01-31

Usage restrictions:

Copyright

Copyright date:

2017

Copyright by:

Springer New York, New York, NY 

Adult content:

No

Language:

English

Categories:

Medicine, Nonfiction